Analysis

CURRENT WORK

A Novel Method of Determination, Validation and Quantification of phenols in human plasma by HPLC

The purpose of the study was to develop an HPLC method to quantify phenols in plasma. Following liquid-liquid extraction, carvacrol and propofol (internal standard) were separated using a binary gradient on a Shim-Pack CLC-C18 column using a mobile phase of acetonitrile/water (40:60, v/v). Samples were eluted at a flow rate of 0.6mL/min with detection wavelength at 274 nm throughout the 14-min run. This analytical method was successfully applied to assess the plasma exposure of phenols in study participants who regularly consume oil of oregano products.

Clinical Study on the Pharmacokinetics of Carvacrol

To determine for the first time the absolute bioavailability of carvacrol, an aromatic monoterpenic phenol, isomeric with thymol, in humans.

When Rheumatoid Arthritis is a Symptom and Not a Disease: Meta-Analysis Points to Misdiagnoses

Meta-analysis demonstrates that antibiotic interventions lead to a reduction in Rheumatoid Arthritis disease activity and reduce acute phase reactants. These antibiotics (minocycline, doxycycline, roxithromycin, clarithromycin) are the same antibiotics which are prescribed for Lyme disease. There is a need for better serological testing protocols initially when symptoms of arthritis present, and a demand for further research into increasingly specific biomarkers for both of these diseases in order to reduce the risk of misdiagnosis that inevitably leads to disease progression.